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il1β  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc il1β
    Il1β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 529 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+rabbit+il1%CE%B2/IL-1beta+Rabbit+mAb/pm41832478-243-17-18
    Average 96 stars, based on 529 article reviews
    il1β - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Lysis:

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis.
    Article Snippet: Dulbecco‘s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; antirabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis
    Article Snippet: Dulbecco`s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; anti-rabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Nucleic Acid Electrophoresis:

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis.
    Article Snippet: Dulbecco‘s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; antirabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis
    Article Snippet: Dulbecco`s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; anti-rabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Membrane:

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis.
    Article Snippet: Dulbecco‘s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; antirabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis
    Article Snippet: Dulbecco`s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; anti-rabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Saline:

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis.
    Article Snippet: Dulbecco‘s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; antirabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.

    Article Title: Double functionalized haemocompatible silver nanoparticles control cell inflammatory homeostasis
    Article Snippet: Dulbecco`s modified Eagle medium (DMEM) has low glucose and 10% heat-inactivated fetal bovine serum from Sigma Aldrich, USA, with an antibiotic concoction (5 mg/mL penicillin, 5 mg/mL streptomycin, and 10 mg/mL neomycin from Gibco Inc, Tokyo, Japan). .. Radioimmunoprecipitation (RIPA) cell lysis buffer from Thermo Scientific, Carlsbad, USA, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels, polyvinylidene fluoride (PVDF) membrane from Immuno Blot, Bio-Rad, California, USA, blotted membrane from EzBlock Chemi, Atto, Corp, Japan, primary antibodies; anti-rabbit IL-6, anti-rabbit TNF-α, anti-rabbit- IL1β, anti-rabbit β-actin from cell signaling Technology Inc, Danvers, MA, USA, tris buffered saline (T-TBS) membranes with horseradish peroxidase (HRP) conjugated secondary antibody, and Enhanced chemiluminescence (ECL) reagents from GE health care, Tokyo, Japan, were procured and used as instructed. .. The Raw 264.7 macrophage cells from RIKEN cell bank of RIEKN cell engineering division, Tsukuba, Ibaraki, Japan; RNAiso reagents from Takara Biolabs, Tokyo, Japan.



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    Image Search Results


    Effects of ERN1 chemical inhibitors and ERN1 knockdown on dust extract induction of inflammatory mediator protein levels in Beas2B bronchial epithelial cells. (A–E). Cells were first treated for 1 h with medium alone, 1 μM KIRA6, or 1 μM APY29 prior to incubation with 1% dust extract for 3 h. (F–K). Cells were transfected with 10 nM non‐targeting control siRNA or ERN1 siRNA and treated with 1% dust extract for 24 h. Cellular pro IL1β, ICAM1, and ERN1 protein levels and secreted IL6 and CXCL8 levels were determined by western blotting and ELISA, respectively. Pro IL1β, ICAM1, and ERN1 protein levels were normalized to Actin levels. Representative western blots are shown (A, F). Data shown are mean ± SE ( n = 4–8 for chemical inhibitor experiments and n = 4–5 for siRNA transfection experiments). Statistical significance was analyzed by two‐way ANOVA followed by Sidak's post hoc test for analysis between specific groups (G) and Tukey's post hoc test for analysis between multiple groups (B–E, H–K). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. C, control; DE, dust extract.

    Journal: FASEB BioAdvances

    Article Title: Endoplasmic Reticulum Stress and Unfolded Protein Response Sensor ERN1 Regulates Organic Dust Induction of Lung Inflammation

    doi: 10.1096/fba.2025-00069

    Figure Lengend Snippet: Effects of ERN1 chemical inhibitors and ERN1 knockdown on dust extract induction of inflammatory mediator protein levels in Beas2B bronchial epithelial cells. (A–E). Cells were first treated for 1 h with medium alone, 1 μM KIRA6, or 1 μM APY29 prior to incubation with 1% dust extract for 3 h. (F–K). Cells were transfected with 10 nM non‐targeting control siRNA or ERN1 siRNA and treated with 1% dust extract for 24 h. Cellular pro IL1β, ICAM1, and ERN1 protein levels and secreted IL6 and CXCL8 levels were determined by western blotting and ELISA, respectively. Pro IL1β, ICAM1, and ERN1 protein levels were normalized to Actin levels. Representative western blots are shown (A, F). Data shown are mean ± SE ( n = 4–8 for chemical inhibitor experiments and n = 4–5 for siRNA transfection experiments). Statistical significance was analyzed by two‐way ANOVA followed by Sidak's post hoc test for analysis between specific groups (G) and Tukey's post hoc test for analysis between multiple groups (B–E, H–K). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. C, control; DE, dust extract.

    Article Snippet: IL1β (D3U3E) , 1:1000 , 12703, Cell Signaling Technology, Danvers, MA, USA.

    Techniques: Knockdown, Incubation, Transfection, Control, Western Blot, Enzyme-linked Immunosorbent Assay